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KMID : 1007520210300050745
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Food Science and Biotechnology
2021 Volume.30 No. 5 p.745 ~ p.753
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Characteristics for phage-encoded cell wall hydrolase of LysSAP27 to reduce staphylococcal food poisoning
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Park Do-Won
Lee Young-Duck
Park Jong-Hyun
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Abstract
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To reduce staphylococcal food poisoning (SFP), a phage-encoded cell wall hydrolase called endolysin has emerged as an attractive antibacterial agent. In this study, the Staphylococcus aureus infecting phage vB_SauS-SAP27 (?SAP27) was isolated from sewage and characterized morphologically and genetically. ?SAP27 was identified as Siphoviridae temperate phage, with a genome of 43 kbp. A ?SAP27 endolysin named LysSAP27 was produced recombinantly in Escherichia coli. LysSAP27 exhibited the highest activity at neutral pH and a temperature of 30 °C, and its lytic activity was upregulated by calcium ions. Following optimization of the enzymatic conditions, LysSAP27 was applied to S. aureus-contaminated milk. Treatment with 2 μM LysSAP27 led to a significant bactericidal effect, corresponding to a reduction in bacterial titer by 2.8 log CFU/mL within 1 h and 3.4 log CFU/mL within 2 h. Therefore, LysSAP27 could be used as an effective antimicrobial agent to prevent SFP in food.
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KEYWORD
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Bacteriophage, Endolysin, Staphylococcus aureus, Food Poisoning
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